Two critical events precede protein synthesis. First, aminoacyl-tRNA synthetases attach amino acids to their respective tRNAs with high specificity through a two-step reaction that begins with the activation of the amino acid using AMP, derived from ATP. Second, ribosomes must dissociate into their subunits at the conclusion of each translation cycle. In bacteria, this dissociation is actively facilitated by RRF and EF-G, while IF3 binds to the free 30S subunit, preventing its reassociation with the 50S subunit to form a complete ribosome.
The initiation codon in prokaryotes is typically AUG but can also be GUG or, more rarely, UUG. The initiating aminoacyl-tRNA is N-formyl-methionyl-tRNAfMet. N-formyl-methionine (fMet) is the first amino acid incorporated into a polypeptide chain, although it is often removed during protein maturation. The 30S initiation complex is formed by the association of a free 30S ribosomal subunit with mRNA and fMet-tRNAfMet. This binding depends on base pairing between the Shine-Dalgarno sequence, located just upstream of the initiation codon in mRNA, and a complementary sequence at the 3'-end of the 16S rRNA. IF3 mediates this interaction with the assistance of IF1 and IF2, which are all bound to the 30S subunit at this stage.
IF2 plays a central role in promoting the binding of fMet-tRNAfMet to the 30S initiation complex, while the other two initiation factors provide essential support. GTP is required for IF2 binding under physiological IF2 concentrations, though it is not hydrolyzed during this process. The complete 30S initiation complex consists of one 30S ribosomal subunit, one molecule each of mRNA, fMet-tRNAfMet, GTP, IF1, IF2, and IF3. GTP hydrolysis occurs after the 50S subunit joins the 30S complex to form the functional 70S initiation complex.