This episode of MCAT Basics covers several separation and purification techniques you may see on the MCAT. We’ll cover these techniques in three distinct categories: small molecules, proteins, and cells.
Within the small molecule category, we’ll discuss extraction, crystallization and recrystallization, and chromatography (TLC and HPLC).
For proteins, we cover SDS-page, precipitation, soelectric focusing, and chromatography variations. Lastly, we’ll explain, within the world of cells, centrifugation, flow cytometry, and microfluidics.
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[00:00] MCAT Tutorig from MedSchoolCoach
[00:34] Welcome to MCAT Basics
[01:07] Topics covered in this episode
[02:24] Techniques for small molecules - extraction, crystallization and recrystallization, and chromatography (TLC and HPLC)
[28:24] Techniques for proteins - SDS-page, precipitation, isoelectric focusing, and chromatography variations
[54:47] Techniques for cells - centrifugation, flow cytometry, and microfluidics.